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human gastric cancer cell line snu 1  (ATCC)


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    ATCC human gastric cancer cell line snu 1
    Human Gastric Cancer Cell Line Snu 1, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 486 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gastric+cancer+cell+lines+snu+1/SNU-1/us12497465-645-0-10
    Average 96 stars, based on 486 article reviews
    human gastric cancer cell line snu 1 - by Bioz Stars, 2026-09
    96/100 stars

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    other:

    Article Title: MiR-148a Functions as a Tumor Suppressor by Targeting CCK-BR via Inactivating STAT3 and Akt in Human Gastric Cancer
    Article Snippet: Gastric cancer cell lines SNU-1 (ATCC: CRL-5971), SNU-16 (ATCC: CRL-5974), AGS (ATCC: CRL-1739), NCI-N87 (ATCC: CRL-5822) and KATOIII (ATCC: HTB-103) were purchased from the American Type Culture Collection (Manassas, VA, USA).

    Article Title: Down-regulation of miR-622 in gastric cancer promotes cellular invasion and tumor metastasis by targeting ING1 gene
    Article Snippet: Gastric cancer cell lines SNU-1 and NCI-N87 were obtained from American Type Culture Collection (Manassas, VA, USA).

    Article Title: Overexpressed miR-301a promotes cell proliferation and invasion by targeting RUNX3 in gastric cancer.
    Article Snippet: Background MicroRNAs can promote or suppress the evolution of malignant behaviors by regulating multiple targets.. We aimed to determine the expression of miR-301a recently screened in gastric cancer, to investigate the biological effects of miR-301a and to identify the specific miR-301a target gene.. Methods Quantitative real-time RT-PCR was used to test miR-301a expression.

    Article Title: microRNA-29c inhibits cell proliferation by targeting NASP in human gastric cancer
    Article Snippet: The human gastric cancer cell lines SNU-1 (ATCC No. CRL-5971), SNU-16 (ATCC No. CRL-5974), NCI-N87 (ATCC No. CRL-5822), AGS (ATCC No. CRL-1739) and KATOIII (ATCC No. HTB-103) were got from the American Type Culture Collection, MKN-45 (JCRB No. 0254) and MKN-28 (JCRB No. 0253) were obtained from the Japanese Cancer Research Resources Bank, and the others (BGC-823: CBP60477, SGC-7901: CBP60500) were obtained from Shanghai Institute for Biological Sciences, Chinese Academy of Science.

    Article Title: Stromal fibroblasts in the microenvironment of gastric carcinomas promote tumor metastasis via upregulating TAGLN expression
    Article Snippet: Gastric cancer cell lines SNU-1 (ATCC: CRL-5971), AGS (ATCC: CRL-1739), NCI-N87 (ATCC: CRL-5822) and KATOIII (ATCC: HTB-103) were obtained from the American Type Culture Collection (Manassas, VA, USA).

    Article Title: VEZT, a novel putative tumor suppressor, suppresses the growth and tumorigenicity of gastric cancer.
    Article Snippet: Gastric cancer cell lines SNU-1 and NCI-N87 cells were obtained from American Type Culture Collection (Manassas, VA, USA).

    Article Title: VEZT, a Novel Putative Tumor Suppressor, Suppresses the Growth and Tumorigenicity of Gastric Cancer
    Article Snippet: Gastric cancer cell lines SNU-1 and NCI-N87 cells were obtained from American Type Culture Collection (Manassas, VA, USA).

    In Vitro:

    Article Title: Long non-coding RNA GATA6-AS inhibits gastric cancer cell proliferation by downregulating microRNA-25-3p
    Article Snippet: .. In order to perform in vitro experiments, gastric cancer cell lines SNU-1 and AGS were purchased from the American Type Culture Collection (ATCC). .. The cells were cultured in RPMI-1640 medium (ATCC) containing 10% fetal bovine serum (Sangon Biotech Co.) and penicillin-streptomycin (100 U/ml) at 37°C with 5% CO 2 .



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    Tumor cell-derived ESM1 and recombinant ESM1 induce specifically endothelial cell responses. ( A ) ( i ) A CCK8 assay analyzed the proliferation of HUVECs treated with the CM of <t>AGS-shNC/AGS-shESM1-1/AGS-shESM1-2</t> and ( ii <t>)</t> <t>HGC27-Vector/HGC27-ESM1</t> stable cell lines. Data are shown as the mean ± SD of triplicate independent sets of experiments; statistical significance was assessed by a paired t -test, * < 0.05, ** < 0.01, and *** < 0.001. ( B ) ( i ) An EDU assay analyzed the proliferation of HUVECs treated with the CM of AGS-shNC/AGS-shESM1-1/AGS-shESM1-2 and ( ii ) HGC27-Vector/HGC27-ESM1 stable cell lines. Representative images ( left panel ) and quantification ( right panel ) are shown as indicated. Data from independent experiments are presented as the mean ± SD. Statistical significance was assessed by an unpaired t -test. ** < 0.01. Scale bar: 50 μm. ( C ) ( i ) A wound healing assay analyzed the migration of HUVECs treated with the CM of AGS-shNC/AGS-shESM1-1/AGS-shESM1-2 and ( ii ) HGC27-Vector/HGC27-ESM1 at 0 and 24 h. Representative images ( left panel ) and quantification ( right panel ) are shown as indicated. Data from independent experiments are presented as the mean ± SD. Statistical significance was assessed by an unpaired t -test. ** < 0.01. Scale bar: 100 μm. ( D ) Transwell migration and Matrigel invasion assays were performed to assess the migration and invasion ability of HUVECs treated with the CM of ( i ) AGS-shESM1-1/AGS-shESM1-2 and ( ii ) HGC27-vector/HGC27-ESM1. Representative images ( left panel ) and quantification ( right panel ) are shown as indicated. Data from independent experiments are presented as the mean ± SD. Statistical significance was assessed by an unpaired t -test. ** < 0.01, *** < 0.001. Scale bar: 50 μm. ( E ) A tube formation assay was performed to assess the tube-forming ability of HUVECs treated with the CM of ( i ) AGS-shESM1-1/AGS-shESM1-2 and ( ii ) HGC27-vector/HGC27-ESM1. Representative images ( left panel ) and quantification ( right panel ) are shown as indicated. Data from independent experiments are presented as the mean ± SD. Statistical significance was assessed by an unpaired t -test. ** < 0.01, *** < 0.001. Scale bar: 200 μm.
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    Tumor cell-derived ESM1 and recombinant ESM1 induce specifically endothelial cell responses. ( A ) ( i ) A CCK8 assay analyzed the proliferation of HUVECs treated with the CM of AGS-shNC/AGS-shESM1-1/AGS-shESM1-2 and ( ii ) HGC27-Vector/HGC27-ESM1 stable cell lines. Data are shown as the mean ± SD of triplicate independent sets of experiments; statistical significance was assessed by a paired t -test, * < 0.05, ** < 0.01, and *** < 0.001. ( B ) ( i ) An EDU assay analyzed the proliferation of HUVECs treated with the CM of AGS-shNC/AGS-shESM1-1/AGS-shESM1-2 and ( ii ) HGC27-Vector/HGC27-ESM1 stable cell lines. Representative images ( left panel ) and quantification ( right panel ) are shown as indicated. Data from independent experiments are presented as the mean ± SD. Statistical significance was assessed by an unpaired t -test. ** < 0.01. Scale bar: 50 μm. ( C ) ( i ) A wound healing assay analyzed the migration of HUVECs treated with the CM of AGS-shNC/AGS-shESM1-1/AGS-shESM1-2 and ( ii ) HGC27-Vector/HGC27-ESM1 at 0 and 24 h. Representative images ( left panel ) and quantification ( right panel ) are shown as indicated. Data from independent experiments are presented as the mean ± SD. Statistical significance was assessed by an unpaired t -test. ** < 0.01. Scale bar: 100 μm. ( D ) Transwell migration and Matrigel invasion assays were performed to assess the migration and invasion ability of HUVECs treated with the CM of ( i ) AGS-shESM1-1/AGS-shESM1-2 and ( ii ) HGC27-vector/HGC27-ESM1. Representative images ( left panel ) and quantification ( right panel ) are shown as indicated. Data from independent experiments are presented as the mean ± SD. Statistical significance was assessed by an unpaired t -test. ** < 0.01, *** < 0.001. Scale bar: 50 μm. ( E ) A tube formation assay was performed to assess the tube-forming ability of HUVECs treated with the CM of ( i ) AGS-shESM1-1/AGS-shESM1-2 and ( ii ) HGC27-vector/HGC27-ESM1. Representative images ( left panel ) and quantification ( right panel ) are shown as indicated. Data from independent experiments are presented as the mean ± SD. Statistical significance was assessed by an unpaired t -test. ** < 0.01, *** < 0.001. Scale bar: 200 μm.

    Journal: Cancers

    Article Title: ESM1 Interacts with c-Met to Promote Gastric Cancer Peritoneal Metastasis by Inducing Angiogenesis

    doi: 10.3390/cancers16010194

    Figure Lengend Snippet: Tumor cell-derived ESM1 and recombinant ESM1 induce specifically endothelial cell responses. ( A ) ( i ) A CCK8 assay analyzed the proliferation of HUVECs treated with the CM of AGS-shNC/AGS-shESM1-1/AGS-shESM1-2 and ( ii ) HGC27-Vector/HGC27-ESM1 stable cell lines. Data are shown as the mean ± SD of triplicate independent sets of experiments; statistical significance was assessed by a paired t -test, * < 0.05, ** < 0.01, and *** < 0.001. ( B ) ( i ) An EDU assay analyzed the proliferation of HUVECs treated with the CM of AGS-shNC/AGS-shESM1-1/AGS-shESM1-2 and ( ii ) HGC27-Vector/HGC27-ESM1 stable cell lines. Representative images ( left panel ) and quantification ( right panel ) are shown as indicated. Data from independent experiments are presented as the mean ± SD. Statistical significance was assessed by an unpaired t -test. ** < 0.01. Scale bar: 50 μm. ( C ) ( i ) A wound healing assay analyzed the migration of HUVECs treated with the CM of AGS-shNC/AGS-shESM1-1/AGS-shESM1-2 and ( ii ) HGC27-Vector/HGC27-ESM1 at 0 and 24 h. Representative images ( left panel ) and quantification ( right panel ) are shown as indicated. Data from independent experiments are presented as the mean ± SD. Statistical significance was assessed by an unpaired t -test. ** < 0.01. Scale bar: 100 μm. ( D ) Transwell migration and Matrigel invasion assays were performed to assess the migration and invasion ability of HUVECs treated with the CM of ( i ) AGS-shESM1-1/AGS-shESM1-2 and ( ii ) HGC27-vector/HGC27-ESM1. Representative images ( left panel ) and quantification ( right panel ) are shown as indicated. Data from independent experiments are presented as the mean ± SD. Statistical significance was assessed by an unpaired t -test. ** < 0.01, *** < 0.001. Scale bar: 50 μm. ( E ) A tube formation assay was performed to assess the tube-forming ability of HUVECs treated with the CM of ( i ) AGS-shESM1-1/AGS-shESM1-2 and ( ii ) HGC27-vector/HGC27-ESM1. Representative images ( left panel ) and quantification ( right panel ) are shown as indicated. Data from independent experiments are presented as the mean ± SD. Statistical significance was assessed by an unpaired t -test. ** < 0.01, *** < 0.001. Scale bar: 200 μm.

    Article Snippet: This research used gastric cancer cell lines AGS, HGC27, MKN45, NCI-N87, and SNU-1, bought from Procell Life Science & Technology Co., Ltd. (Wuhan, China); HEK293T and human gastric mucosal epithelial cell line GES-1 were all stored in Dr. Zhou’s laboratory under standard conditions.

    Techniques: Derivative Assay, Recombinant, CCK-8 Assay, Plasmid Preparation, Stable Transfection, EdU Assay, Wound Healing Assay, Migration, Tube Formation Assay

    Tumor cell-derived ESM1 promotes angiogenesis and GC metastasis. ( A ) Images of peritoneal metastasis in nude BALB/c mice after injection. Arrows point to typical examples. ( B ) Statistical significance of the peritoneal nodes’ weights. *** < 0.001. ( C ) Number of peritoneal metastases obtained from mice in each group. *** < 0.001. ( D ) Ascites volume of mice in each group. ** < 0.01, *** < 0.001. ( E ) Number of mice with or without ascitic fluid in each group. * < 0.05. ( F ) ( i ) A CAM assay was performed to assess angiogenic ability after being treated with the CM of AGS-shESM1-1/AGS-shESM1-2 and HGC27-vector/HGC27-ESM1. ( ii ) A CAM assay was performed to assess angiogenic ability after being treated with CM mixed with recombinant ESM1 or neutralizing antibody against ESM1 and the CM of AGS-shNC mixed with a neutralizing antibody against ESM1. The inner diameter of the sampling rings was 8 mm. ( G ) A Matrigel Plug assay was performed to assess angiogenic ability by post-subcutaneous injection of matrigel mixed with either shNC CM or shESM1 CM from AGS stable cells or the vector CM or ESM1 CM from HGC27 stable cells ( i ) or recombinant ESM1 (100 ng/mL) or ESM1 Ab or IgG (negative control) ( ii ). Representative images ( left ) and hemoglobin quantification ( right ). Data from independent experiments are presented as the mean ± SD. Statistical significance was assessed by an unpaired t -test. ** < 0.01, *** < 0.001.

    Journal: Cancers

    Article Title: ESM1 Interacts with c-Met to Promote Gastric Cancer Peritoneal Metastasis by Inducing Angiogenesis

    doi: 10.3390/cancers16010194

    Figure Lengend Snippet: Tumor cell-derived ESM1 promotes angiogenesis and GC metastasis. ( A ) Images of peritoneal metastasis in nude BALB/c mice after injection. Arrows point to typical examples. ( B ) Statistical significance of the peritoneal nodes’ weights. *** < 0.001. ( C ) Number of peritoneal metastases obtained from mice in each group. *** < 0.001. ( D ) Ascites volume of mice in each group. ** < 0.01, *** < 0.001. ( E ) Number of mice with or without ascitic fluid in each group. * < 0.05. ( F ) ( i ) A CAM assay was performed to assess angiogenic ability after being treated with the CM of AGS-shESM1-1/AGS-shESM1-2 and HGC27-vector/HGC27-ESM1. ( ii ) A CAM assay was performed to assess angiogenic ability after being treated with CM mixed with recombinant ESM1 or neutralizing antibody against ESM1 and the CM of AGS-shNC mixed with a neutralizing antibody against ESM1. The inner diameter of the sampling rings was 8 mm. ( G ) A Matrigel Plug assay was performed to assess angiogenic ability by post-subcutaneous injection of matrigel mixed with either shNC CM or shESM1 CM from AGS stable cells or the vector CM or ESM1 CM from HGC27 stable cells ( i ) or recombinant ESM1 (100 ng/mL) or ESM1 Ab or IgG (negative control) ( ii ). Representative images ( left ) and hemoglobin quantification ( right ). Data from independent experiments are presented as the mean ± SD. Statistical significance was assessed by an unpaired t -test. ** < 0.01, *** < 0.001.

    Article Snippet: This research used gastric cancer cell lines AGS, HGC27, MKN45, NCI-N87, and SNU-1, bought from Procell Life Science & Technology Co., Ltd. (Wuhan, China); HEK293T and human gastric mucosal epithelial cell line GES-1 were all stored in Dr. Zhou’s laboratory under standard conditions.

    Techniques: Derivative Assay, Injection, Chick Chorioallantoic Membrane Assay, Plasmid Preparation, Recombinant, Sampling, Matrigel Assay, Negative Control

    ESM1 interacts with membrane receptor c-Met and promotes pro-angiogenic factor expression. Predicted proteins could interact with ESM1 based on the STRING ( A ) and GENEMANIA ( B ) database. ( C ) The Venn showed overlapped proteins. ( D ) The binding complex models of ESM1 and c-Met were predicted by using the Cluspro online protein docking tool. ( E ) HUVECs were treated with recombinant ESM1 for 2 h, followed by double immunofluorescence staining with c-Met (green) ESM1 (red) antibodies. Nuclei were counterstained with DAPI (blue). ( F ) The immunoprecipitation of the ESM1 protein by an anti-c-Met antibody and the immunoprecipitation of the c-Met protein by an anti-ESM1 antibody. IgG was used as a negative control. The uncropped blots are shown in . ( G ) Immunoprecipitation of the c-Met protein by an anti-Flag antibody in HUVECs (transfected with Myc-c-Met) treated with the CM of HEK293 cells transfected with Flag-ESM1. The uncropped blots are shown in . ( H ) GST pulldown was performed to determine the direct interaction between ESM1 and c-Met. Recombinant GST and GST-ESM1 were purified from bacteria and analyzed by SDS-PAGE and Coomassie blue staining. ( I ) The fold change of classic pro-angiogenic factor expression in HUVECs treated with recombinant ESM1. ns: no significance; * < 0.05 and *** < 0.001. ( J ) The Western blot analyzed pro-angiogenic factors showed protein expression in HUVECs treated with the CM of AGS-shNC/AGS-shESM1-1/AGS-shESM1-2 and HGC27-Vector/HGC27-ESM1. The uncropped blots are shown in . ( K ) The Western blot analysis of lysates from HUVECs starved for 6 h and then incubated with recombinant ESM1 for up to 60 min, followed by the immunoblotting of lysates The uncropped blots are shown in . ( L ) The EDU assay and its quantification ( M ) are shown as indicated. Data from independent experiments are presented as the mean ± SD. Statistical significance was assessed by an unpaired t -test. ** < 0.01. Scale bar: 50 μm. ( N ) The Transwell assay and its quantification ( O ) are shown as indicated. Data from independent experiments are presented as the mean ± SD. Statistical significance was assessed by an unpaired t -test. ** < 0.01. Scale bar: 50 μm. and ( P ) Tube formation and its quantification ( Q ) are shown as indicated. Data from independent experiments are presented as the mean ± SD. Statistical significance was assessed by an unpaired t -test. ** < 0.01. Scale bar: 50 μm. All experiments were performed in the absence or presence of recombinant ESM1. Graph of the average number of meshes as described in .

    Journal: Cancers

    Article Title: ESM1 Interacts with c-Met to Promote Gastric Cancer Peritoneal Metastasis by Inducing Angiogenesis

    doi: 10.3390/cancers16010194

    Figure Lengend Snippet: ESM1 interacts with membrane receptor c-Met and promotes pro-angiogenic factor expression. Predicted proteins could interact with ESM1 based on the STRING ( A ) and GENEMANIA ( B ) database. ( C ) The Venn showed overlapped proteins. ( D ) The binding complex models of ESM1 and c-Met were predicted by using the Cluspro online protein docking tool. ( E ) HUVECs were treated with recombinant ESM1 for 2 h, followed by double immunofluorescence staining with c-Met (green) ESM1 (red) antibodies. Nuclei were counterstained with DAPI (blue). ( F ) The immunoprecipitation of the ESM1 protein by an anti-c-Met antibody and the immunoprecipitation of the c-Met protein by an anti-ESM1 antibody. IgG was used as a negative control. The uncropped blots are shown in . ( G ) Immunoprecipitation of the c-Met protein by an anti-Flag antibody in HUVECs (transfected with Myc-c-Met) treated with the CM of HEK293 cells transfected with Flag-ESM1. The uncropped blots are shown in . ( H ) GST pulldown was performed to determine the direct interaction between ESM1 and c-Met. Recombinant GST and GST-ESM1 were purified from bacteria and analyzed by SDS-PAGE and Coomassie blue staining. ( I ) The fold change of classic pro-angiogenic factor expression in HUVECs treated with recombinant ESM1. ns: no significance; * < 0.05 and *** < 0.001. ( J ) The Western blot analyzed pro-angiogenic factors showed protein expression in HUVECs treated with the CM of AGS-shNC/AGS-shESM1-1/AGS-shESM1-2 and HGC27-Vector/HGC27-ESM1. The uncropped blots are shown in . ( K ) The Western blot analysis of lysates from HUVECs starved for 6 h and then incubated with recombinant ESM1 for up to 60 min, followed by the immunoblotting of lysates The uncropped blots are shown in . ( L ) The EDU assay and its quantification ( M ) are shown as indicated. Data from independent experiments are presented as the mean ± SD. Statistical significance was assessed by an unpaired t -test. ** < 0.01. Scale bar: 50 μm. ( N ) The Transwell assay and its quantification ( O ) are shown as indicated. Data from independent experiments are presented as the mean ± SD. Statistical significance was assessed by an unpaired t -test. ** < 0.01. Scale bar: 50 μm. and ( P ) Tube formation and its quantification ( Q ) are shown as indicated. Data from independent experiments are presented as the mean ± SD. Statistical significance was assessed by an unpaired t -test. ** < 0.01. Scale bar: 50 μm. All experiments were performed in the absence or presence of recombinant ESM1. Graph of the average number of meshes as described in .

    Article Snippet: This research used gastric cancer cell lines AGS, HGC27, MKN45, NCI-N87, and SNU-1, bought from Procell Life Science & Technology Co., Ltd. (Wuhan, China); HEK293T and human gastric mucosal epithelial cell line GES-1 were all stored in Dr. Zhou’s laboratory under standard conditions.

    Techniques: Membrane, Expressing, Binding Assay, Recombinant, Double Immunofluorescence Staining, Immunoprecipitation, Negative Control, Transfection, Purification, Bacteria, SDS Page, Staining, Western Blot, Plasmid Preparation, Incubation, EdU Assay, Transwell Assay